The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 21, 2002

Filed:

Sep. 18, 2000
Applicant:
Inventors:

Foster T. Jordan, Hollywood, SC (US);

Hui-Ti Chiang, Charleston, SC (US);

James F. Cooper, Charleston, SC (US);

Norman R. Wainwright, Falmouth, MA (US);

Assignee:

Charles River Laboratories, Wilmington, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 3/3554 ; G01N 3/353 ; A61K 3/902 ; A61K 4/500 ;
U.S. Cl.
CPC ...
G01N 3/3554 ; G01N 3/353 ; A61K 3/902 ; A61K 4/500 ;
Abstract

The invention provides methods and compositions for the detection and/or quantification of bacterial endotoxins. In particular, provided herein is an inexpensive and reproducible method for producing an improved amebocyte lysate preparation having reduced Factor G activity. Provided also is an endotoxin-specific amebocyte lysate preparation produced by such a method. In addition, the invention provides methods and compositions akin for enhancing the sensitivity to endotoxins of amebocyte lysate preparations having reducing Factor G activity. In particular, the sensitivity of such amebocyte lysate preparations to endotoxins can be enhanced by the addition of exogenous (1→3) &bgr;-D-glucan.


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