The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 07, 2002
Filed:
Jan. 22, 1998
Jean Content, Rhode-Saint-Genese, BE;
Kris Huygen, Brussels, BE;
Margaret A. Liu, Rosemont, PA (US);
Donna Montgomery, Chalfont, PA (US);
Jeffrey Ulmer, Chalfont, PA (US);
Merck & Co., Inc., Rahway, NJ (US);
Abstract
Genes encoding ( ) proteins were cloned into eukaryotic expression vectors to express the encoded proteins in mammalian muscle cells in vivo. Animals were immunized by injection of these DNA constructs, termed polynucleotide vaccines or PNV, into their muscles. Immune antisera was produced against antigens. Specific T-cell responses were detected in spleen cells of vaccinated mice and the profile of cytokine secretion in response to antigen 85 was indicative of a T 1 type of helper T-cell response (i.e., high IL-2 and IFN-&ggr;). Protective efficacy of an DNA vaccine was demonstrated in mice after challenge with BCG, as measured by a reduction in mycobacterial multiplication in the spleens and lungs of DNA-vaccinated mice compared to control DNA-vaccinated mice or primary infection in naive mice.