The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 07, 2002
Filed:
Aug. 24, 1999
Applicant:
Inventors:
John D. Mountz, Birmingham, AL (US);
Huang-Ge Zhang, Birmingham, AL (US);
Assignee:
UAB Research Foundation, Birmingham, AL (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 7/01 ; C12N 1/586 ; C12N 1/5861 ;
U.S. Cl.
CPC ...
C12N 7/01 ; C12N 1/586 ; C12N 1/5861 ;
Abstract
The present invention discloses a method of producing large-scale recombinant adeno-associated virus stocks by infection of cells with at least one recombinant adenovirus vector(s). The vector(s) encode a therapeutic gene flanked by the terminal repeat ends of adeno-associated virus and the adeno-associated virus rep and cap genes. Transfection with two recombinant adenovirus vector(s) instead of two plasmids plus adenovirus results in the large scale, high titer production of recombinant adeno-associated virus with little to no contaminating adenovirus present.