Anthocyanin producing callus line in cultures of panax sikkimensis and a method of producing panax sikkimensis line capable of producing anthocyanin

Abstract

The present invention relates to an anthocyanin producing callus line in cultures of Panax sikkimensis (an Indian species of ginseng) said line comprising: (a) Characteristic pink-purple pigmentation, (b) Growth index of about 221-450 in about 50-80 days of culture period, (c) Yield of anthocyanin (2-3 mg/gf.wt.) from the callus in about 40-60 days under light conditions (continuous light), and (d) Characteristic DNA profile wherein the lanes 1,2,3,4,5,6,7,8,9,10,11 and 12 in each gel (upper gel for wild line and lower gel for anthocyanin producing line) represent the PCR amplified fragments produced by the respective template megabase genomic DNA with primers 5′ CTG ATG CAT C3′, 3′,5′ TGG TCA CTG A 3′, 5′ AGG GGT CTT G 3′,5′ GAA ACG GGT G 3′,5′ AGG GGT CTT G 3′,5′ GCG TAA CGC C 3′,5′ CAG CAC CCA C 3′,5′ GTT GCG ATC C 3′,5′ CAG GCC CTT C 3′,5′ CGC AGT ACT C 3′,5′ GTC CTA CTC G 3′,5′ CTA CAC AGG C 3′ and 5′ GTC CTT AGC G 3′, respectively. Lane M shows the standard size markers of a ladder at 100 bp intervals from 1000 bp downwards.