The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 02, 2002
Filed:
Mar. 28, 2000
Sunil Kumar Chattopadhyay, Lucknow, IN;
Suchitra Banerjee, Lucknow, IN;
Shipra Agarwal, Lucknow, IN;
Koneni Venkata Sashidhara, Lucknow, IN;
Vinayak Tripathi, Lucknow, IN;
Arun Kumar Kukreja, Lucknow, IN;
Sushil Kumar, Lucknow, IN;
Manish Kulshrestha, Lucknow, IN;
Ram Prakash Sharma, Lucknow, IN;
Vijay Kumar Mehta, Lucknow, IN;
Council of Scientific and Industrial Research, New Delhi, IN;
Abstract
This invention relates to a process for the production of a compound (+) catechin penta acetate of formula I from tissue cultures which comprises : (a) inoculation of explants on different media compositions supplemented with combinations of auxins (1-5 mg/l) and cytokinins (0.1-1.0 mg/l); (b) incubation of the cultures under continuous light or dark conditions for 4-6 weeks for callus cultures followed by subculturing at 4-6 weeks intervals; (c) harvesting of the calli at different growth phases ranging from 12-36 months; (d) extraction of fresh pulverized calli with polar solvents at room temperature; (e) evaporating the solvent to give a residue; and partitioning of the residue between water and chlorinated solvents and evaporating the solvent to a semisolid mass; (f) subjecting the resultant mass to column chromatography over suitable adsorbent and (g) eluting with organic solvent/mixtures of organic solvents to get (+) catechin penta acetate.