The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 26, 2002
Filed:
Apr. 01, 1998
Applicant:
Inventors:
Jeffrey Van Ness, Seattle, WA (US);
John C. Tabone, Bothell, WA (US);
Lori K. Garrison, Seattle, WA (US);
Assignee:
QIAGEN Genomics, Inc., Bothell, WA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C07H 1/900 ; C07H 2/100 ; C07H 2/102 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C07H 1/900 ; C07H 2/100 ; C07H 2/102 ; C07H 2/104 ;
Abstract
Compositions and methods are provided for increasing the specificity of a probe nucleic acid for a target nucleic acid in a hybridization solution. An abasic residue, deoxyNebularine residue, or a hybotrope is used to increase specificity. A method is provided for identifying useful hybotropes, including salts, water miscible organic solvents, aprotic solvents and organic solvents, on the basis of enthalpy considerations. Hybotropic hybridization and modified oligonucleotides may be used in amplification reactions, such as PCR, sequence analysis methods, and genomic screening methods.