The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 19, 2002
Filed:
Nov. 12, 1998
Abe Fumiyoshi, Yokosuka, JP;
Koki Horikoshi, Yokosuka, JP;
Japan Marine Science and Technology Center, Yokosuka, JP;
Abstract
A method for detecting microorganisms present in a sample comprises the steps of: applying a non-lethal hydrostatic pressure to a sample containing microorganisms; and staining the microorganisms with a fluorescent dye. The method permits a significant increase in the uptake of a fluorescent substance by applying, to a sample, a desired non-lethal hydrostatic pressure, without causing any reduction of the survival rate of microorganisms. The method also permits the elimination of such a secondary effect that the subject microorganism undergoes proliferation during staining the same with a fluorescent dye. Thus, the present invention would contribute to the determination of the correct viable count in a wide variety of technical fields.