The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 05, 2002
Filed:
Jul. 28, 1999
Jay A. Tischfield, Carmel, IN (US);
Jeffrey J. Seilhamer, Los Altos Hills, CA (US);
Other;
Abstract
Novel mammalian phospholipase (PLA ) nucleotide sequences and low molecular weight (about 14 KD) amino acid sequences encoded thereby are disclosed. More particularly, a cloned human HPLA cDNA expressing HPLA -10 and its cloned rat RPLA cDNA counterpart, expressing RPLA -10, which are characterized as PLA Type IV, are disclosed. A second type of PLA cDNA, characterized as PLA Type III and exemplified by a rat PLA cDNA encoding RPLA -8 and a partial human PLA nucleotide sequence encoding HPLA -8, is disclosed. Expression of the cDNAs encode the two new types of PLA enzymes which have phospholipase activity. The novel PLA s do not include disulfide bridges between cysteine amino acids 11 and 77 or elapid loops. However, the novel PLA s may include amino acid COOH-terminal extensions which can vary in length. Seventeen of the eighteen absolutely conserved amino acids in all active 14 KD PLA s are believed to be conserved in RPLA -8 and HPLA -8, whereas all eighteen are believed to be conserved in RPLA -10 and HPLA -10. Because the encoded sequences of RPLA -8 and HPLA -8 include only 16 cysteine amino acids, they have been designated as Type III. RPLA -10 and HPLA -10 are designated as Type IV since their encoded sequences include only 12 cysteine amino acids.