The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 15, 2002

Filed:

Feb. 28, 2000
Applicant:
Inventors:

Yuji Hatada, Tochigi, JP;

Kazuaki Igarashi, Tochigi, JP;

Katsuya Ozaki, Tochigi, JP;

Katsutoshi Ara, Tochigi, JP;

Shuji Kawai, Tochigi, JP;

Susumu Ito, Tochigi, JP;

Assignee:

Kao Corporation, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/28 ; C12N 1/21 ; C12N 9/26 ; C12N 1/552 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12N 9/28 ; C12N 1/21 ; C12N 9/26 ; C12N 1/552 ; C07H 2/104 ;
Abstract

The present invention provides a DNA fragment encoding alkaline pullulanase exhibiting alkaline &agr;-amylase activity, alkaline &agr;-amylase possessing both an amino acid sequence described in SEQ ID NO:3 and a DNA fragment encoding the amylase, alkaline pullulanase possessing both an amino acid sequence described in SEQ ID NO:4 and a DNA fragment encoding the pullulanase, recombinant DNAs containing these DNA fragments, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting alkaline &agr;-amylase activity.


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