The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 25, 2001
Filed:
Jul. 27, 1993
Applicant:
Inventors:
Rudolf Glockshuber, Adlmannstein, DE;
Martina Wunderlich, Regensburg, DE;
Arne Skerra, Wiesbaden, DE;
Rainer Rudolph, Weilheim, DE;
Assignee:
Roche Diagnostics GmbH, Mannheim, DE;
Primary Examiner:
Int. Cl.
CPC ...
C12P 2/104 ;
U.S. Cl.
CPC ...
C12P 2/104 ;
Abstract
The invention concerns a process for increasing the formation of the natural protein conformation when disulfide-bonded proteins are secreted by a prokaryotic host organism that contains a recombinant DNA coding for the secreted protein whereby the host organism is cultured in a suitable culture medium under suitable conditions for the expression of the recombinant DNA, which is characterized in that a culture medium containing 0.1 to 20 mmol/l of one or several thiol reagents is used.