The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 18, 2001
Filed:
Nov. 04, 1998
Nobuo Kato, Kameoka, JP;
Ajinomoto Co., Inc., Tokyo, JP;
Abstract
Hexulose phosphate synthase was purified from,to determine an amino acid sequence of the enzyme. Oligonucleotide primers to be used for PCR were synthesized on the basis of obtained amino acid sequence information. A DNA fragment, which was amplified by performing PCR by using a template of genomic DNA prepared from,, was used as a probe so that colony hybridization was carried out with respect to a library comprising fragments obtained by digesting,genomic DNA with PstI to obtain a positive clone. Thus, the DNA fragment which contains ORF encoding hexulose phosphate synthase was obtained. Furthermore, the DNA fragment cloned contained other ORF(ORF-1). The expression system of ORF-1 was constructed (pT-HPIS-1), and it was introduced into,cell. After the induction of the expression of ORF-1, the hexulose phosphate isomerase activity was found in the extract of cells harvoring pT-HPIS-1. Thus, gene, which coded for hexulose phosphate isomerase, was obtained together with ORF which coded for hexulose phosphate synthase.