The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 13, 2001
Filed:
Dec. 23, 1999
Reid D. Frederick, Ashburn, VA (US);
Paul W. Tooley, Braddock Heights, MD (US);
Morris R. Bonde, Middletown, MD (US);
David A. Knorr, San Leandro, CA (US);
Gary L. Peterson, Frederick, MD (US);
Norman W. Schaad, Myersville, MD (US);
The United States of America as represented by the Secretary of Agriculture, Washington, DC (US);
Abstract
Karnal bunt of wheat is caused by,Mitra. Recently, teliospores morphologically resembling,were isolated from bunted ryegrass seeds and wheat seed washes. Previously developed PCR assays failed to differentiate,from the newly discovered ryegrass pathogen,,. The nucleotide sequence of a 2.3-kb region of mitochondrial DNA, previously amplified by PCR only from,, was determined for three isolates of,and for three isolates of,. There was greater than 99% identity within either the,group or the,group of isolates, whereas there was approximately 3% divergence between isolates of these two Tilletia species. Five sets of PCR primers were made specific to,, and three sets were designed specifically for,based upon nucleotide differences within the mtDNA region. In addition, a 212 bp amplicon was developed as a target sequence in a fluorogenic 5′ nuclease PCR assay using the TaqMan system for the detection and discrimination of,and