The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 30, 2001
Filed:
Apr. 20, 2000
Dorothee Ambrosius, Munich, DE;
Rainer Rudolph, Halle, DE;
Joerg Schaeffner, Halle, DE;
Elisabeth Schwarz, Halle, DE;
Hoffmann-La Roche Inc., Nutley, NJ (US);
Abstract
A process produces a water-soluble, naturally folded eukaryotic polypeptide containing two or several cysteines linked by disulfide bridges. This process involves culturing prokaryotic cells, a) in which the prokaryotic cells contain an expression vector which encodes the polypeptide which contains a prokaryotic signal sequence at the N-terminus, b) under conditions under which the polypeptide is secreted into the periplasm or the medium, c) cleaving the signal sequence and isolating the polypeptide from the periplasm or the medium. In this process, the culturing is carried out in the presence of arginine or a compound of the formula I R,—CO—NR,(I) in which R and R,represent hydrogen or a saturated or unsaturated branched or unbranched C,-C,alkyl chain and R,represents hydrogen, NHR,or a saturated or unsaturated branched or unbranched C,-C,alkyl chain, is suitable for the recombinant production of polypeptides in prokaryotes in a high yield.