The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 30, 2001
Filed:
Mar. 30, 2000
Applicant:
Inventors:
Kevin Raney, Little Rock, AR (US);
Patrick Morris, Little Rock, AR (US);
Regina Dennis, Little Rock, AR (US);
Assignee:
Board of Trustees of the University of Arkansas, Little Rock, AR (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; G01N 3/353 ; C12P 1/934 ; C12N 9/00 ; C07H 2/102 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; G01N 3/353 ; C12P 1/934 ; C12N 9/00 ; C07H 2/102 ;
Abstract
The gp4l and Dda helicases were found to significantly enhance the dissociation rate of streptavidin from biotin-labeled oligonucleotides in an ATP dependent reaction, demonstrating that these enzymes are capable of imparting a significant force on a molecule blocking their path. The present invention describes an assay for studying enzymatic activity of a helicase using the rate of dissociation of streptavidin from biotinylated oligonucleotides.