The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 23, 2001
Filed:
Apr. 28, 1999
Applicant:
Inventors:
Alex Pelletier, Fabreville, CA;
Chris M. Farnet, Outremont, CA;
Assignee:
Boehringer Ingelheim (Canada) Ltd., Laval, CA;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/70 ; C12Q 1/68 ; G01N 3/353 ;
U.S. Cl.
CPC ...
C12Q 1/70 ; C12Q 1/68 ; G01N 3/353 ;
Abstract
The present invention relates to a method for isolating cloned papillomavirus E1 protein from a eukaryotic expression system having demonstrable and reproducible viral helicase activity and preparation containing essentially pure E1 protein. The invention further relates to the use of this novel E1 protein preparation in a screening assay for identifying antiviral agents. More particularly a high throughput assay to screen for agents capable of inhibiting HPV DNA replication. The assay is based on measuring the effect of antiviral agents on the activity of the E1 protein and more specifically on its helicase activity.