The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 02, 2001

Filed:

Jan. 28, 2000
Applicant:
Inventors:

Todd E. French, Cupertino, CA (US);

Douglas N. Modlin, Palo Alto, CA (US);

John C. Owicki, Palo Alto, CA (US);

James S. Richey, Palo Alto, CA (US);

Lev J. Leytes, Palo Alto, CA (US);

Enal S. Razvi, San Francisco, CA (US);

Assignee:

LJL BioSystems, Inc., Sunnyvale, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C02Q 1/68 ; C12P 1/934 ; C07M 2/102 ;
U.S. Cl.
CPC ...
C02Q 1/68 ; C12P 1/934 ; C07M 2/102 ;
Abstract

Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) allele-specific hybridization, using luminescence detection, (2) allele-specific oligonucleotide ligation, using dye-labeled oligonucleotide ligation with luminescence resonance energy transfer (FRET) detection, and (3) allele-specific nucleotide incorporation, using primer extension with luminescence polarization (FP) detection. More specifically, the methods may include (1) locating a sample containing a nucleic acid material at an examination site, (2) illuminating the sample, (3) detecting light transmitted from the sample, and (4) determining the presence, absence, and/or identity of a nucleic acid target in the sample using the light transmitted from the sample.


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