The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 18, 2001
Filed:
Nov. 17, 1995
Applicant:
Inventor:
Andrew John Garman, Chester, GB;
Assignee:
Zeneca Limited, London, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/37 ; C12N 9/48 ; C12N 9/50 ; G01N 3/352 ;
U.S. Cl.
CPC ...
C12Q 1/37 ; C12N 9/48 ; C12N 9/50 ; G01N 3/352 ;
Abstract
A method for the preparation of a fluorescence resonance energy transfer (FRET) substrate having donor and acceptor species on opposite sides of a proteolytic cleavage site and wherein the donor and/or acceptor species are attached via the side chain(s) of amino acid(s) therein. The method comprises contacting a reactive donor or acceptor species with a polypeptide substrate having the side chain(s) of amino acid(s) therein adapted for reaction with the reactive species and then contacting the substrate so obtained with a corresponding reactive donor or acceptor species. Novel FRET substrates so prepared and their use in assays to identify modulators of protease activity.