The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 04, 2001
Filed:
Feb. 14, 1997
Applicant:
Inventors:
Assignee:
Boehringer Ingelheim International GmbH, Ingelheim am Rhein, DE;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07H 2/104 ; C07K 1/00 ; C12N 1/500 ; C12N 1/563 ;
U.S. Cl.
CPC ...
C07H 2/104 ; C07K 1/00 ; C12N 1/500 ; C12N 1/563 ;
Abstract
The toxicity problems which occur when foreign material is introduced into higher eukaryotic cells, particularly during transfection with DNA, are countered by causing gene products to be expressed in the cell, which block the apoptosis triggered by the transfection process, and/or by treating the cells with anti-inflammatory substances. Preferably, Bcl-2, E1B 19K or an anti-apoptotically active gene of chicken adenovirus CELO is used as the anti-apoptosis gene whilst the inflammatory substance used is adenovirus VA1, which is introduced into the cell in the form of VA1-DNA. Using these methods, long-lasting gene expression can be achieved.