Selective purification and enrichment sorting of flow cytometer droplets based upon analysis of droplet precursor regions

Abstract

A flow cytometer sorting mechanism is operative to controllably sort successively generated fluid droplets in accordance with the contents of a plurality of contiguous precursor regions of the carrier fluid which are contained within a target droplet of interest and precursor regions for droplets on either side of the target droplet. This multi-region sorting window scheme is particularly useful in reducing the time required to harvest a highly purified quantity of a particular type of cell, as not only are droplets containing only desired cells sorted to a highly purified collection store, but should an undesired cell be detected within a predefined proximity of a desired cell, rather than being simply aborted, the droplet is sorted into an auxiliary enrichment container, whose contents may then be reclaimed for resorting. Since all droplets are sorted to one of a plurality of droplet reclamation containers, substantially no detected/target cells are lost. As a result, through dual collection and resorting of the contents of the enrichment container the total harvest time can be significantly reduced.