The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 28, 2001
Filed:
May. 26, 1999
Appu Rathinavelu, Fort Lauderdale, FL (US);
Andres Malave, Fort Lauderdale, FL (US);
Nova Southeastern University, Fort Lauderdale, FL (US);
Abstract
The present invention is a simple, selective, rapid and a non-radioactive procedure for estimating the activity of protein kinases and protein phosphatases. The method involves attaching a suitable substrate to a 96-well plate surface and then measuring the activity of the specific enzymes using suitable incubating conditions. The PKC assay involves immobilizing histone (type III-SS), the substrate for PKC, and then determining the extent of phosphorylation by using ammonium molybdate and 1-amino-2-naphthol-4-sulfonic acid based reaction for inorganic phosphate quantitation. The PP-1 assay involves immobilizing phosphorylase-b substrate to the assay plate and then phosphorylating the substrate using phosphorylase kinase. Finally, the phosphate is hydrolyzed from the substrate by protein phosphatase. The assay methods are suitable for estimating PKC and PP-1 activity in tissue and cell samples without using any radiolabeled substrate.