The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 21, 2001

Filed:

Dec. 23, 1998
Applicant:
Inventors:

Yoshio Jo, Chiba-ken, JP;

Toshiki Inoue, Kashiwa, JP;

Kouichi Takada, Fujinomiya, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 2/100 ; G01N 3/3563 ; G01N 3/3543 ; C12M 1/00 ; A61K 3/800 ;
U.S. Cl.
CPC ...
G01N 2/100 ; G01N 3/3563 ; G01N 3/3543 ; C12M 1/00 ; A61K 3/800 ;
Abstract

Disclosed is a colorimetric sensor comprising polydiacetylene membrane liposomes, a polydiacetylene membrane film or fine particles coated with a polydiacetylene membrane, in which the polydiacetylene membrane is incorporated with a protein having a reduced molecular weight low enough not to cause color change in the polydiacetylene membrane. The examples of the reduced-molecular-weight proteins include an antibody Fab′ fragment, an antigenic protein of molecular weight of 100,000 or less, and a peptide consisting of 3-20 amino acid residue, which undergo an antigen-antibody reaction with an antigen or antibody contained in a sample. As the reduced-molecular-weight protein is also employed a combination of single-stranded DNA of 100 bases or less which hybridizes with single-stranded DNA contained in a sample to form a double-stranded DNA, and an antibody which reacts with said double-stranded DNA but does not react with the single-stranded DNA contained in the sample. A method for analysis of biosample is also disclosed, which comprises contacting the colorimetric sensor with a solution sample and utilizing an absorption measurement or a visual observation with the naked eye to detect color change in the polydiacetylene membrane.


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