Optical fiber having dye-labeled cytochrome c′ immobilized on a fiber tip

Abstract

Fiber-optic sensors and fiberless sensors are made for measuring analytes, in particular nitric oxide. The sensors contain a compound specific for the analyte such a nitric oxide-binding compound. Fiber-optic sensors contain the compound immobilized on the tip of the fiber. The tip may be coated with an inert coating such as a metal layer and the compound is immobilized on the coating. Nitric oxide-binding compounds include heme-binding proteins, porphyrin group-containing proteins, heme group-containing proteins, dye-labeled porphyrin group-containing proteins and dye-labeled heme group-containing proteins. In a specific embodiment, dye-labeled cytochrome c′ such as fluorescein-labeled cytochrome c′ is immobilized on a fiber tip containing a gold colloid layer. The fiberless sensors are small enough to enter a single mammalian cell relatively non-invasively. Embodiments of the sensors include a polymer fiberless sensor containing a nitric oxide-binding compound and a polymer such as polyacrylamide, a sol-gel fiberless sensor containing a nitric oxide-binding compound entrapped in a matrix and a metal fiberless sensor containing a nitric oxide-binding compound immobilized on metal particles such as metal colloids.