The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 26, 2001
Filed:
Oct. 21, 1999
Applicant:
Inventor:
Michael J. Adang, Madison, WI (US);
Assignee:
Mycogen Plant Science, Inc., San Diego, CA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/20 ; C12N 1/500 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12N 1/20 ; C12N 1/500 ; C07H 2/104 ;
Abstract
The,var. kurstaki HD-73 crystal protein gene was cloned into pBR322.,cells harboring this recombinant plasmid produced a 130 kD protoxin that was toxic to,(tobacco hornworm) larvae. Plasmids having the 3′-end of the protoxin gene deleted where also constructed.,cells harboring these deleted plasmids produced an active, soluble 68 kD toxin, provided that the 3′-deletion had not removed sequences encoding the 68 kD toxin. The invention provides methods to produce 68 kD toxin protein by constructing partial protoxin genes encoding the toxin followed by expression of the genes in living cells. Useful plasmids and cells are also provided.