The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 19, 2001

Filed:

Dec. 23, 1998
Applicant:
Inventors:

Jim C. Spain, Panama City, FL (US);

Shirley F. Nishino, Panama City, FL (US);

Urs Lendenmann, Rehetobel, CH;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12S 1/300 ;
U.S. Cl.
CPC ...
C12S 1/300 ;
Abstract

Dinitrotoluene present as a contaminant in a sample, is degraded by the steps of (a) providing a sample comprising dinitrotoluene; (b) adding to the sample at least one bacterial strain capable of degrading at least one dinitrotoluene isomer under aerobic conditions; (c) producing aerobic conditions in the sample; and (d) maintaining the aerobic conditions in the sample for a time that is sufficient for the bacteria to degrade said dinitrotoluene. In one embodiment, at least one bacterial strain capable of degrading the 2,4-dinitrotoluene isomer under aerobic conditions is added to the sample. In another embodiment, at least one bacterial strain capable of degrading the 2,6-dinitrotoluene isomer under aerobic conditions is added to the sample. In yet another embodiment, at least one bacterial strain capable of degrading both the 2,4-dinitrotoluene isomer and the 2,6-dinitrotoluene isomer under aerobic conditions is added to the sample. In a further embodiment, a mixed culture of bacteria that degrade both isomers under aerobic conditions is added to the sample.


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