The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 12, 2001
Filed:
Dec. 17, 1999
Alison Velyain Todd, Hondi Beach, AU;
Caroline Jane Fuery, Balga, AU;
Johnson & Johnson Research Pty. Limited, St. Leonards, AU;
Abstract
The present invention provides a method for amplifying a specific target nucleic acid sequence. The method comprises (1) forming a reaction mixture comprising: (i) the target sequence; (ii) primers comprising a first primer at least a portion of which at the 3′ end thereof is substantially complementary to a first segment at a first end of the target sequence, a second primer at least a portion of which at the 5′ end thereof is substantially complementary to a second segment at a second end of the target sequence, the 5′ end of the second primer being adjacent the 3′ end of the first primer, and a third primer, the third primer being substantially complementary to a segment of the second primer at the 3′ end thereof; (iii) at least four different nucleotide bases; (iv) thermostable polymerase and thermostable ligase; and (2) thermocycling the reaction mixture.