The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 05, 2001

Filed:

Oct. 01, 1999
Applicant:
Inventors:

Sydney G. Kustu, Berkeley, CA (US);

Volker F. Wendisch, Juelich, DE;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C12Q 1/48 ; C12N 9/12 ; C12N 1/20 ; C12N 1/509 ; C07H 2/100 ; C07H 2/102 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C12Q 1/48 ; C12N 9/12 ; C12N 1/20 ; C12N 1/509 ; C07H 2/100 ; C07H 2/102 ;
Abstract

The invention provides methods and compositions for selectively isolating total bacterial mRNA. The general method comprises contacting a bacterial lysate comprising total bacterial mRNA and nonisolated bacterial polysomes with an exogenous enzyme under conditions wherein the enzyme selectively modifies the mRNA to form modified mRNA, and isolating the modified mRNA. In particular embodiments, the enzyme is selected from a poly(A) polymerase, a RNA ligase and a terminal deoxynucleotidyl transferase. Depending on the enzyme, the modified mRNA may have any of a variety of modifications, such as a 3′ tail, particularly a poly(A) tail, a specific sequence tag, a detectably labeled nucleotide, etc.


Find Patent Forward Citations

Kustu, Sydney. (2001). Selective isolation of bacterial mrna (U.S. Patent No. 6242189). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/6242189

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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