The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 29, 2001
Filed:
Jun. 16, 1999
Robert Cornelis Hoeben, Leiden, NL;
Abraham Bout, Moerkapelle, NL;
Introgene B.V., Leiden, NL;
Abstract
A method for intracellular amplification of DNA is disclosed. The method includes providing a mammalian cell with a first nucleic acid sequence encoding functional adenoviral E2A and E2B gene products and with a second nucleic acid sequence encoding a linear DNA fragment to be amplified. The second nucleic acid sequence further has at least one functional adenoviral Inverted Terminal Repeat on a terminus and, in one embodiment where there is only a single ITR, a hairpin-like structure on the other terminus. This allows the linear DNA fragment to be acted upon by the adenoviral E2A and E2B gene products, thus intracellularly amplifying the linear DNA fragment, which can be extracted.