The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 15, 2001

Filed:

Aug. 09, 1999
Applicant:
Inventors:

Chiranjit Deka, Miami, FL (US);

Mark A. Wells, Davie, FL (US);

Carlos M. Rodriguez, Miami, FL (US);

Carlos Reyes, Miami, FL (US);

Assignee:

Coulter International Corp., Miami, FL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 3/348 ;
U.S. Cl.
CPC ...
G01N 3/348 ;
Abstract

A method and apparatus for differentiating and enumerating the five major sub-populations of leukocytes in a blood sample (i.e., lymphocytes, monocytes, eosinophils, neutrophils and basophils) uses multiangle light scatter and DC (Coulter) volume measurements. Light scattering characteristics of the leukocytes are determined within five different angular ranges, all being lower than 40 degrees. The invention is particularly useful in differentiating and enumerating the basophil sub-population which has heretofore required more complex apparatus and/or chemical processing.


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