The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 08, 2001

Filed:

Dec. 09, 1999
Applicant:
Inventors:

Toshio Ichikawa, Chiba, JP;

Yasuji Koyama, Chiba, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/06 ;
U.S. Cl.
CPC ...
C12N 9/06 ;
Abstract

This invention relates to sarcosine oxidase having the following physico-chemical properties: (a) action: oxidatively hydrolyzing 1 mole sarcosine to give 1 mole glycine, 1 mole formaldehyde, and 1 mole hydrogen peroxide; (b) substrate specificity: specific for sarcosine; (c) optimum pH: 7.0-8.0; (d) stable pH range: 7.0-9.5; (e) suitable temperature range for action: 50° C.; (f) thermostability: 55° C. or less; and (g) molecular weight: 44,000 daltons (when estimated roughly from the amino acid sequence of the wild-type), and to a process for producing the sarcosine oxidase, comprising the steps of culturing a microorganism having an ability to produce the sarcosine oxidase and collecting the sarcosine oxidase from the culture are provided.


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