The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 24, 2001
Filed:
Mar. 24, 1999
James E. Hopper, Hershey, PA (US);
The Penn State Research Foundation, University Park, PA (US);
Abstract
The invention provides methods and recombinant expression constructs for inducing and sustaining high-level production of a recombinant polypeptide in yeast. The invention specifically provides high copy number recombinant expression constructs that express high levels of trans-acting transcription factors that in turn induce expression of a recombinant nucleic acid encoding a heterologous or endogenous recombinant polypeptide. The invention more specifically provides constructs that express galactose-inducible and temperature-sensitive transcription factors. The invention also provides constructs comprising nucleic acids the transcription of which is regulated by the transcription factors expressed by the construct. The invention also provides yeast cells transformed by the recombinant expression constructs of the invention that permit sustained high-level expression of a recombinant polypeptide. The invention further provides methods for producing yeast cells transformed with the recombinant expression constructs of the invention, and methods for inducing high-level expression of a recombinant polypeptide in said transformed yeast cells. The invention also provides such recombinant polypeptides produced by the methods and the transformed yeast cells of the invention.