The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 17, 2001
Filed:
May. 04, 1999
Applicant:
Inventors:
John K. Critser, Carmel, IN (US);
Erik J. Woods, Indianapolis, IN (US);
Assignee:
General Biotechnology, LLC, Carmel, IN (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01N 1/02 ; C07G 1/700 ; C07K 1/00 ;
U.S. Cl.
CPC ...
A01N 1/02 ; C07G 1/700 ; C07K 1/00 ;
Abstract
A method to remove cryoprotectants from cryopreserved biological cells and tissues is described. Enzymes are used to convert compounds used as cryoprotectants for cells during cryopreservation to membrane impermeable products. This process effectively removes the cryoprotectant chemicals from the cells prior to their use in transfusion, transplantation, insemination or other applications, without causing osmotic damage due to cell swelling. Methods are described for subsequent removal of the enzymes and enzyme conversion products from the cell and tissue preparations.