The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 27, 2001
Filed:
Oct. 13, 1999
Applicant:
Inventors:
Yasuhiro Mihara, Kawasaki, JP;
Takashi Utagawa, Tokyo, JP;
Hideaki Yamada, Kyoto, JP;
Yasuhisa Asano, Imizu-gun, JP;
Assignee:
Ajinomoto Co., Inc., Tokyo, JP;
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/16 ; C12N 9/12 ;
U.S. Cl.
CPC ...
C12N 9/16 ; C12N 9/12 ;
Abstract
A method for producing nucleoside-5′-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phospahte group donor using an acid phosphatase having an increased affinity for the nucleoside and/or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5′-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and/or an enhanced temperature stability are also provided.