The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 27, 2001
Filed:
Dec. 21, 1994
Craig W. Adams, Corona, CA (US);
Beckman Coulter, Inc., Fullerton, CA (US);
Abstract
Disclosed herein are methods for the detection and exponential amplification of a target nucleic acid sequence. In a preferred embodiment, the methodology relies upon three oligonucleotide moieties: a Blocker moiety capable of hybridizing to a target sequence; a Primer moiety capable of hybridizing to the target sequence in an adjacent fashion to the Blocker moiety; and an End-Run moiety having a sequence which is complementary to the Blocker moiety. A ligation event between the hybridized Blocker and Primer moieties, followed by elongation of the End-Run moiety along the ligated Blocker and Primer moieties, provides multiple copies of target sequence such that during cyclical amplification, exponential amounts of the original target sequence are provided.