The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 20, 2001
Filed:
Sep. 29, 1998
Karsten Hellmuth, Marklohe, DE;
Rual Lopez-Ulibarri, Montclair, NJ (US);
Anne Françoise Mayer, New York, NY (US);
Heinrich Winfried Schlieker, Bloomfield, NJ (US);
Adolphus van Loon, Rheinfelden, CH;
Roche Vitamins Inc., Parsippany, NJ (US);
Abstract
A method for producing a protein by culturing a cell capable of expressing the protein and which contains a methylotrophic yeast promoter for an enzyme of the methanol metabolic pathway controlling expression of the protein, in a fermentative batch process comprising a batch phase and a feeding phase, under conditions such that dissolved oxygen is continually present in the culture medium throughout the process, and about 1% to about 100% of the carbon source during the feeding phase is a sugar or sugar polymer, which sugar or sugar polymer is provided in such an amount that the sugar or sugar polymer is continually depleted by the cell and therefore is substantially undetectable in the culture medium; and isolating the protein on completion of the feeding phase.