The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 27, 2001
Filed:
Mar. 26, 1999
Applicant:
Inventor:
Kenneth A. Cruickshank, Naperville, IL (US);
Assignee:
Vysis, Inc., Downers Grove, IL (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C07H 1/900 ; C07H 2/100 ; C07H 2/102 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C07H 1/900 ; C07H 2/100 ; C07H 2/102 ; C07H 2/104 ;
Abstract
The invention relates to a method for linking a detectable label to a nucleic acid by (1) providing a nucleic acid bound to a solid support, the nucleic acid having a cytidine base; (2) transaminating the cytidine base with a reactive group to form a covalent linkage between the cytidine base and the reactive group; and (3) linking a detectable label to the reactive group. The invention also includes compositions containing a labeled nucleic acid produced by the methods of the invention immobilized on a solid support, and a kit containing a solid support, a bisulfite, a reactive group, and a detectable label.