The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 13, 2001
Filed:
Aug. 06, 1998
Thermolabile uracil-dna-glycosylas, process for its preparation and use for removing uracil from dna
Applicant:
Inventors:
Harald Sobek, Penzberg, DE;
Manfred Schmidt, Penzberg, DE;
Bruno Frey, Penzberg, DE;
Klaus Kaluza, Bad Heilbrunn, DE;
Assignee:
Roche Diagnostics GmbH, Mannheim, DE;
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/14 ;
U.S. Cl.
CPC ...
C12N 9/14 ;
Abstract
Thermolabile enzyme with uracil-DNA-glycosylase activity which is in particular characterized by a high degree of purity, short half-lives and a content of contaminating foreign activities of less than 2%, a process for its isolation as well as the use thereof to remove the base uracil from DNA and in particular from PCR products containing uracil. The enzyme is obtainable from gram-positive microorganisms such as e.g. Arthrobacter or Micrococcus.