The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 13, 2001

Filed:

Nov. 03, 1999
Applicant:
Inventors:

Derk Jon Bergsma, Berwyn, PA (US);

Jeffrey L Mooney, Limerick, PA (US);

Assignee:

SmithKline Beecham Corporation, Philadelphia, PA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C12P 1/934 ; C12N 9/00 ; C07H 2/102 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C12P 1/934 ; C12N 9/00 ; C07H 2/102 ; C07H 2/104 ;
Abstract

The present invention provides a method for high throughput cloning of full length cDNA sequences using a pooling strategy. This method uses a plurality of clone arrays prepared from cDNA libraries which have been preferably enriched for 5 ′ mRNA sequences and size fractionated into several discrete ranges (sub-libraries). These arrays are used to rapidly identify the full length cDNA sequence for a DNA segment of interest.


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