The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 30, 2001
Filed:
Jun. 14, 1999
Gregory J. Brewer, Springfield, IL (US);
Paul J. Price, Grand Island, NY (US);
The Board of Trustees of Southern Illinois University, Springfield, IL (US);
Abstract
The present invention provides a minimal essential medium for maintaining neural cell or tissue viability in an environment containing ambient levels of CO,. The medium contains less than about 2000 &mgr;M bicarbonate, a buffer having a pKa of from about 6.9 to about 7.7, wherein the medium is free of ferrous sulfate, glutamate and aspartate, from 0 to about 3000 &mgr;M CaCl,, from about 0.05 to about 0.8 &mgr;M Fe(NO,),, from about 2500 to about 10000 &mgr;M KCl, from 0 to about 4000 &mgr;M MgCl,, from about 74000 to about 103000 &mgr;M NaCl, from about 400 to about 2000 &mgr;M NaHCO,, from about 250 to about 4000 &mgr;M NaH,PO,, from about 0.2 to about 2 &mgr;M ZnSO,, from about 2500 to about 50000 &mgr;M glucose and from about 23 to about 500 &mgr;M sodium pyruvate. The present invention also provides a process of extending neural cell or tissue viability in an atmosphere having ambient levels of carbon dioxide whereby the neural cells or tissue are placed in such a medium. Preferably, the medium is supplemented with a growth-promoting medium that contains effective amounts of hormones, essential fatty acids and anti-oxidants for neural cells.