The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 16, 2001
Filed:
Mar. 11, 1998
Applicant:
Inventors:
Bernd Kirschbaum, Mainz, DE;
Wilhelm Stahl, Idstein, DE;
Irvin Winkler, Liederbach, DE;
Michael Meisterernst, Eichenau, DE;
Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/563 ; C12N 1/564 ; C12Q 1/68 ; G01N 3/353 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12N 1/563 ; C12N 1/564 ; C12Q 1/68 ; G01N 3/353 ; C07H 2/104 ;
Abstract
An process for analyzing transcription which can be automated and which is suitable for bulk screening. The process involves transcribing a DNA sequence using a nuclear extract, which can be complemented or fully replaced by exogenous transcription factors and/or cofactors; optionally removing the proteins of the reaction mixture; binding the resulting transcript to a solid matrix; removing the excess labeled nucleotides; and determining the amount of labeled transcript. Methods of using the inventive process to identify compounds having a selective effect on gene expression.