The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 16, 2001
Filed:
Jun. 16, 1997
Candida cytoplasmic tryptophanyl-trna synthetase proteins, nucleic acids and strains comprising same
Xiaoyu Shen, Boston, MA (US);
Fariba Houman, Belmont, MA (US);
Cubist Pharmaceuticals, Inc., Cambridge, MA (US);
Abstract
The present invention relates to isolated and/or recombinant nucleic acids which encode Candida cytoplasmic tryptophanyl-tRNA synthetases, portions thereof, or fusion proteins comprising a Candida cytoplasmic tryptophanyl-tRNA synthetase or portion thereof. Also disclosed are constructs comprising the nucleic acids of the present invention, host cells comprising a recombinant nucleic acid or construct, and methods of producing a Candida cytoplasmic tryptophanyl-tRNA synthetase, portion thereof, or fusion protein comprising the same. Also described are tester strains, which are cells engineered to rely on the function of a Candida cytoplasmic tryptophanyl-tRNA synthetase or functional fragment thereof encoded by an introduced cloned gene, and which can be used in a method of detecting an inhibitor of Candida cytoplasmic tryptophanyl-tRNA synthetase function. The invention further relates to isolated and/or recombinant Candida cytoplasmic tryptophanyl-tRNA synthetases, portions thereof, or fusion proteins comprising a Candida cytoplasmic tryptophanyl-tRNA synthetase or portion thereof, methods of use of these polypeptides in an assay to identify inhibitors of Candida cytoplasmic tryptophanyl-tRNA synthetase function, and antibodies reactive with Candida cytoplasmic tryptophanyl-tRNA synthetases.