The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 09, 2001
Filed:
Apr. 07, 1999
Alex Zhu, New York, NY (US);
New York Blood Center, Inc., New York, NY (US);
Abstract
A method for detecting and measuring exoglycosidase activity is presented. The method employs derivatives containing the fluorescent group 4-methylumbelliferyl (“4-Mu”) at a pH lower than that conventionally employed. While the fluorescence intensity due to the 4-Mu group is considerably diminished at the lower pHs employed, the fluorescence intensity is still sufficient to continuously measure exoglycosidase activity in the activity range commonly assayed. The method is easily adaptable to high throughput enzyme assay systems and automated data analysis method. The method also provides a means to detect alterations in exoglycosidase activity that are independent of expression levels.