The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 02, 2001
Filed:
Oct. 21, 1997
Applicant:
Inventors:
Assignee:
Seikagaku Kogyo Kabushiki Kaisha, Tokyo, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 3/353 ; G01N 1/00 ; C12Q 1/37 ; C12N 9/99 ;
U.S. Cl.
CPC ...
G01N 3/353 ; G01N 1/00 ; C12Q 1/37 ; C12N 9/99 ;
Abstract
The presence of endotoxin in the outer membrane of cell wall of a Gram-negative bacteria is used to determine bacterial contamination in a biological product. The amount of endotoxin present in a biological product is accurately measured without influence of a limulus reaction-activating substance which causes a false-positive reaction, by a method including the steps of: inactivating the limulus reaction-activating substance, if any, in the biological product, by exposing the biological product to a surfactant at a temperature ranging from the surfactant's freezing point to,° C.; and measuring the amount of endotoxin present in the biological product, using a limulus reagent.