The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 26, 2000
Filed:
May. 19, 1999
Applicant:
Inventors:
Shigeru Fukushima, Otsu, JP;
Naoko Takaoka, Kameoka, JP;
Assignee:
Shimadzu Corporation, Kyoto, JP;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ; C07H / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 435 912 ; 536 231 ; 536 243 ; 536 2432 ; 536 2433 ; 536 266 ;
Abstract
The object of the present invention is to provide a simple, rapid and highly sensitive method of examining EHEC or VTEC in the examination of food poisoning and diarrhea. In the present invention, the oligonucleotides of SEQ ID NOS: 1-9 hybridizing selectively with a Vero toxin gene from EHEC (or VTEC) or with an O antigen-synthesizing region gene from pathogenic E. coli O157 are prepared and used as primers for gene amplification. By this method, bacteria producing O157 as one of the pathogenic factors in these bacteria and E. coli capable of producing Vero toxin can be selectively detected.