The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 21, 2000
Filed:
Sep. 26, 1997
Jean-Pierre Perreault, Fleurimont, CA;
Daniel LaFontaine, St.-Alexis-de-Montcalm, CA;
Stephane Mercure, Sherbrooke, CA;
Universite de Sherbrooke, Sherbrooke, CA;
Abstract
The present invention relates to an arbitrarily primed PCR-based method to identify genetic marker associated with a pathology, which comprises the steps of: a) collecting heterogeneous nucleic acid samples from diseased and healthy tissues; b) determining quantities of the nucleic acid pools by specific amplification of a fragment of glyceraldehyde-phosphate dehydrogenase (GAPDH) mRNA; c) amplifying nucleic acid pools of step b) using non-specific sense and antisense primers to obtain clear patterns of nucleic acid sequences; and d) subjecting amplified nucleic acid sequences to gel electrophoresis to obtain fingerprints of both diseased and healthy, wherein bands predominantly associated with diseased tissues are markers for the pathology. The present invention also relates to a RNA expression marker for the diagnosis of Crohn's disease, which comprises a 3.1 kb RNA sequence, and uses thereof.