The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 31, 2000
Filed:
Jun. 05, 1998
Nancy A Reichert, Starkville, MS (US);
Vanishree Rudraswamy, Starkville, MS (US);
Mississippi State University, Mississippi State, MS (US);
Abstract
Maize tissue may be regenerated from nodal extracts prepared from germinated mature seeds and germinated embryos. Nodal section explants are secured from seedlings in 3-5 days. The explants are grown on an induction medium until adventitious shoot formation is observed. The shoots are separated and elongated on an MS-based medium, and then rooted. Fast genotype-independent regeneration is obtained, in 12-14 weeks. These explants, as well as zygotic embryos, may be transformed with exogenous DNA using a biolistic approach, where DNA precipitated onto tungsten microprojectiles is accelerated as 650 psi towards the explants at a distance of at least 7.5 microns. Improved frequency of transformation is obtained using microprojectiles which prior to DNA precipitation were frozen in glycerol, and suspending from a preparation of 2.5 M CaCl.sub.2. The combination of transformation process and regeneration can be used, independent of genotype, to provide new commercial crop organisms.