The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 31, 2000
Filed:
Oct. 07, 1998
Achilles A Demetriou, Bel Aire, CA (US);
Andreas Kamlot, Los Angeles, CA (US);
Jacek Rozga, West Lake Village, CA (US);
Cedars-Sinai Medical Center, Los Angeles, CA (US);
Abstract
A method is provided for preconditioning and cryopreservation of cells harvested from a donor. Cells are suspended in a cell conditioning and cryopreservation medium containing a cryopreservative such as Dimethyl Sulfoxide (DMSO) and the suspension is incubated for a period of at least ten minutes and the cells are frozen. The medium includes adenosine, a calcium channel blocker, and a cell nutrient matrix comprising a sufficient amount of cell nutrients to sustain the metabolic needs of the cells during incubation without producing detectable levels of lactate or substantially depleting the nutrient substrates to maintain viability of the harvested cells. In some embodiments, a cryopreservative is added step-wise before the cell suspension is frozen and removed step-wise after the cell suspension is thawed.