The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 31, 2000
Filed:
Jan. 29, 1997
Chia-Cheng Chang, Okemos, MI (US);
James Trosko, Okemos, MI (US);
Michigan State University, East Lansing, MI (US);
Abstract
A method is described for culturing morphologically and antigenically distinguishable types of normal human breast epithelial cells (HBEC) derived from reduction mammoplasty. Type I HBEC showed luminal and stem cell characteristics i.e. the ability to form budding/ductal structures when cultured on MATRIGEL and expressed a variant ER (.about.48 Kd) which has a deletion in the DNA binding domain (exon 2). In contrast, Type II HBEC with basal epithelial phenotype are ER-negative when cultured on MATRIGEL. Simian virus 40 (SV40) transformed Type I and Type II HBEC lines also expressed the variant ER. Tumors formed in athymic nude mice by in vitro transformed tumorigenic Type I cell lines, however, expressed a high level of wild type ER which was undetectable in these cells grown in vitro before and after tumor formation. Thus, there appears to be a differentional ER mRNA splicing between the in vitro and in vivo milieu.
Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile