The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 31, 2000
Filed:
Feb. 23, 1999
Stephen C Wardlaw, Old Saybrook, CT (US);
Wardlaw Partners, LL.P., Lyme, CT (US);
Other;
Abstract
A method and apparatus for determining the minimum inhibitory concentration of an antibiotic for a target microorganism is provided. The method includes the steps of: (a) providing a microorganism growth medium; (b) providing a sensible reagent, which includes an antibiotic mixed with a marker, the marker having a signal with a magnitude proportional to the marker's concentration; (c) incorporating the reagent into the growth medium, in a manner that creates a gradient of concentrations of the antibiotic and marker within the growth medium; (c) inoculating the growth medium with the target microorganism; (d) incubating the inoculated growth medium for a period of time sufficient for the target microorganism to grow a detectable amount on a first section of growth medium; (e) determining a growth boundary between the first section of growth medium having detectable target microorganism growth and a second section having substantially no detectable target microorganism growth; (f) measuring the signal magnitude at the growth boundary; and (g) determining a minimum inhibitory concentration of the antibiotic using the measured signal magnitude.