The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 24, 2000
Filed:
Jan. 19, 1999
Minoru Fukuda, San Diego, CA (US);
Jiunn-Chern Yeh, La Jolla, CA (US);
The Burnham Institute, La Jolla, CA (US);
Abstract
The present invention provides a substantially pure C2GnT-M polypeptide or a functional fragment or derivative thereof, wherein C2GnT-M is characterized as a polypeptide having core 2, core 4 and I branching .beta.-1.fwdarw.6-N-acetylglucosaminyltransferase activities. The invention also provides a substantially pure C2GnT-M peptide, wherein the peptide is immunogenic. Also provided is a method of modifying an acceptor molecule by contacting the acceptor molecule with a substantially pure C2GnT-M polypeptide or a functional fragment under conditions that allow addition of core 2, core 4 or I GlcNAc linkages to the acceptor molecule, and an acceptor molecule produced by the method. Also provided is a substantially pure nucleic acid molecule having substantially the nucleic acid sequence designated SEQ ID NO: 1, or the complement thereof. The invention also provides a substantially pure nucleic acid molecule encoding C2GnT-M or a functional fragment or derivative thereof, or the complement of the nucleic acid molecule, wherein C2GnT-M is characterized as a polypeptide having core 2, core 4 and I branching P-1.fwdarw.6-N-acetylglucosaminyltransferase activities. Also provided are vectors and host cells containing nucleic acid molecules encoding C2GnT-M or a functional fragment or derivative thereof. A substantially pure oligonucleotide having a nucleotide sequence corresponding to or complementary to at least 15 nucleotides from SEQ ID NO: 1, SEQ ID NO: 9 or SEQ ID NO: 13 is also provided. The invention further provides an antibody or antigen binding fragment thereof that is specifically reactive with C2GnT-M or with a functional fragment or derivative hereof. Methods of detecting the presence of C2GnT-M in sample are also provided.