The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 08, 2000
Filed:
Feb. 12, 1998
Liying Wu, Sacramento, CA (US);
Raymond L Rodriguez, Davis, CA (US);
Applied Phytologics, Inc., Sacramento, CA (US);
Abstract
A method for stably transforming barley from mature barley seeds as starting material is disclosed. The method involves germinating mature barley seeds until early shoot development occurs, exposing scutellar or embryo tissue cells on the embryo side of germinated seeds, and introducing foreign DNA into the cells. The cells are initially grown under conditions that allow expression of a selectable marker introduced with the foreign DNA, then on a callus-growth medium effective to suppress callus formation in the absence of the selectable marker. Successfully transformed calli can be cultured in suspension to obtain a desired foreign protein, or regenerated into plants, to obtain the foreign protein from the transformed plants, e.g., germinated seeds.